Naphthalene plasmids in pseudomonads.

Pseudomonads Rule Degradation of Polyaromatic Hydrocarbons in Aerated Sediment

Given that the degradation of aromatic pollutants in anaerobic environments such as sediment is generally very slow, aeration could be an efficient bioremediation option. Using stable isotope probing (SIP) coupled with pyrosequencing analysis of 16S rRNA genes, we identified naphthalene-utilizing populations in aerated polyaromatic hydrocarbon (PAH)-polluted sediment. The results showed that na...

Metabolism of naphthalene by pseudomonads: salicylaldehyde as the first possible inducer in the metabolic pathway.

Pseudomonas ATCC 17483 produced enzymes for naphthalene metabolism when growing in a medium containing succinate and naphthalene. Mutants for naphthalene metabolism produced by treatment with N-methyl-N'-nitro-N-nitrosoguanidine were able to produce these enzymes only when the metabolic pathway was intact as far as salicylaldehyde, which was therefore identified as the first possible inducer.

Oxidative metabolism of naphthalene by soil pseudomonads. The ring-fission mechanism.

Plasmids responsible for horizontal transfer of naphthalene catabolism genes between bacteria at a coal tar-contaminated site are homologous to pDTG1 from pseudomonas putida NCIB 9816-4

The presence of a highly conserved nahAc allele among phylogenetically diverse bacteria carrying naphthalene-catabolic plasmids provided evidence for in situ horizontal gene transfer at a coal tar-contaminated site (J. B. Herrick, K. G. Stuart-Keil, W. C. Ghiorse, and E. L. Madsen, Appl. Environ. Microbiol. 63:2330-2337, 1997). The objective of the present study was to identify and characterize...

Effect of 2-hydroxybenzoate on the maintenance of naphthalene-degrading pseudomonads in seeded and unseeded soil.

The addition of specific nontoxic inducers of catabolic operons to contaminated sites is an approach that may enhance the efficiency of in situ biodegradation. We determined the genetic response of six pseudomonads to salicylate (also known as 2-hydroxybenzoate) added directly to 50 g of nonsterile soil samples. The strains, isolated from a polyaromatic hydrocarbon-contaminated soil, metabolize...